Anti-Glypican 1/ GPC1 antibody (ab226855)
Key features and details
- Rabbit polyclonal to Glypican 1/ GPC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glypican 1/ GPC1 antibody
See all Glypican 1/ GPC1 primary antibodies -
Description
Rabbit polyclonal to Glypican 1/ GPC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rhesus monkey -
Immunogen
Recombinant fragment within Human Glypican 1/ GPC1 (internal sequence). The exact sequence is proprietary.
Database link: P35052 -
Positive control
- WB: A431 and MCF7 whole cell extracts. IHC-P: Human normal pancreas and pancreatic cancer tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Lanes 1-4 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution
Lanes 5-6 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract
Lane 3 : A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract
Lane 4 : A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 6 : MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.