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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker

Anti-Glypican 1/ GPC1 antibody (ab226855)

Anti-Glypican 1/ GPC1 antibody (ab226855)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Glypican 1/ GPC1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Glypican 1/ GPC1 antibody
    See all Glypican 1/ GPC1 primary antibodies
  • Description

    Rabbit polyclonal to Glypican 1/ GPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Glypican 1/ GPC1 (internal sequence). The exact sequence is proprietary.
    Database link: P35052

  • Positive control

    • WB: A431 and MCF7 whole cell extracts. IHC-P: Human normal pancreas and pancreatic cancer tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • HSPGs
    • Cancer
    • Signal transduction
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration

Images

  • Western blot - Anti-Glypican 1/ GPC1 antibody (ab226855)
    Western blot - Anti-Glypican 1/ GPC1 antibody (ab226855)
    Lanes 1-4 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/1000 dilution
    Lanes 5-6 : Anti-Glypican 1/ GPC1 antibody (ab226855) at 1/500 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : A431 cells treated with 24 µg/ml Tunicamycin for 16 hours, whole cell extract
    Lane 3 : A431 cells treated with 4 mM Benzyl glucosinolate for 32 hours, whole cell extract
    Lane 4 : A431 cells treated with both 24 µg/ml Tunicamycin and 4 mM Benzyl glucosinolate, whole cell extract
    Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 6 : MCF7 cells treated with Glycipan 1/GPC1 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (ab226855)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (ab226855)

    Paraffin-embedded human pancreatic cancer tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (ab226855)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody (ab226855)

    Paraffin-embedded human pancreatic tissue stained for Glypican 1/ GPC1 with ab226855 at 1/250 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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