Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Nesprin1/Syne-1 antibody [EPR14196]
See all Nesprin1/Syne-1 primary antibodies
Description

Rabbit monoclonal [EPR14196] to Nesprin1/Syne-1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, 293, HACAT, RAW 264.7, PC12 and NIH 3T3 cell lysates; human kidney, human bladder transitional cell carcinoma and rat stomach tissues; A673 and HACAT cells.
General notes
Previously labelled as Nesprin 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14196
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Immunofluorescent analysis of A673 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Nesprin1/Syne-1 with ab192234 at 1/50 dilution (4.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution; counter-stained with DAPI (blue).
Negative controls: anti-Nesprin1/Syne-1 at 1/50 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.
Flow Cytometry - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Flow cytometric analysis of HACAT cells (paraformaldehyde-fixed, 2%) labeling Nesprin1/Syne-1 with ab192234 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Nesprin1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Nesprin 1/Syne-1 with ab192234 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

All lanes : Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/1000 dilution

Lane 1 : RAW 264.7 cell lysate
Lane 2 : PC12 cell lysate
Lane 3 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 112 kDa
Western blot - Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

All lanes : Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) at 1/20000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HACAT cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 112 kDa
Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com