Human DAND5 ELISA Kit (ab267598)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DAND5 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 142.8 134% - 149% Plasma 128.6 92% - 144% Cell culture media 98.66 92% - 111% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human DAND5 ELISA Kit is designed for the quantitative determination of DAND5 (COCO) in cell culture supernatants, plasma and serum samples.
Δ Note: Human DAND5 concentration is low in normal serum and plasma samples and may not be detectable in this assay.
This assay employs an antibody specific for human DAND5 coated on a 96-well plate. Standards and samples are pipetted into the wells and DAND5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DAND5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DAND5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
No cross-reactivity with human ActivinA, BMP4, DAN or mouse DAND5 is observed.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human DAND5 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human DAND5 Detection Antibody 2 vials Human DAND5 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Function
Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation. -
Sequence similarities
Belongs to the DAN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BMP antagonist 3
- CER2
- cerberus 2
see all -
Database links
- Entrez Gene: 199699 Human
- Entrez Gene: 199699 Human
- Omim: 609068 Human
- SwissProt: Q8N907 Human
- SwissProt: Q8N907 Human
- Unigene: 331981 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.