Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)
![Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)](https://www.abcam.com/ps/products/240/ab240653/Images/ab240653-330766-anti-enpp1-antibody-epr22262-75-western-blot.jpg "Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22262-75] to ENPP1/PC1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ENPP1/PC1 antibody [EPR22262-75]
See all ENPP1/PC1 primary antibodies -
Description
Rabbit monoclonal [EPR22262-75] to ENPP1/PC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney tissue lysate; MDA-MB-231, HepG2 and Huh7 whole cell lysates. Flow cyt: HepG2 cells.
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General notes
This product was previously labelled as ENPP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22262-75 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)All lanes : Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 19577557).
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![Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)](https://www.abcam.com/ps/products/240/ab240653/Images/ab240653-330765-anti-enpp1-antibody-epr22262-75-flow-cytometry.jpg)
Flow Cytometry - Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling ENPP1/PC1 with ab240653 at 1/50 (red) compared with a Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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![Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)](https://www.abcam.com/ps/products/240/ab240653/Images/ab240653-3-benefits-of-recombinant-antibodies.png)
Anti-ENPP1/PC1 antibody [EPR22262-75] (ab240653)
