Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631]
    See all Rac1 + Rac2 + Rac3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18631] to Rac1 + Rac2 + Rac3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human Rac1, Rac2 and Rac3 full length protein. Human fetal brain lysate. HEK-293, HeLa, HUVEC, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18631

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

    Lane 1 : Recombinant Human Rac1 full length protein
    Lane 2 : Recombinant Human Rac2 full length protein
    Lane 3 : Recombinant Human Rac3 full length protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lanes 1 & 2:1 second.

    Lane 3: 3 minutes.

    Recombinant human Rac1 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac2 full length protein contains aa1-192 with a C-terminal His-Tag®; Recombinant human Rac3 full length protein contains aa1-192 with a C-terminal His-Tag®. All three recombinant Rac’s were made in house.

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2-4 : 15 seconds.

  • Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    Western blot - Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    All lanes : Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)
    Anti-Rac1 + Rac2 + Rac3 antibody [EPR18631] (ab180683)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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