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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-RAB3B antibody [EPR12987] (ab177949)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-RAB3B antibody [EPR12987] (ab177949)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12987] to RAB3B
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-RAB3B antibody [EPR12987]
    See all RAB3B primary antibodies
  • Description

    Rabbit monoclonal [EPR12987] to RAB3B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB3B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P20337

  • Positive control

    • 293T, HepG2, fetal brain and SW480 lysates; SW480 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12987
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes

Images

  • Flow Cytometry - Anti-RAB3B antibody [EPR12987] (ab177949)
    Flow Cytometry - Anti-RAB3B antibody [EPR12987] (ab177949)

    Flow cytometric analysis of permeabilized SW480 cells labeling RAB3B with ab177949 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Western blot - Anti-RAB3B antibody [EPR12987] (ab177949)
    Western blot - Anti-RAB3B antibody [EPR12987] (ab177949)
    All lanes : Anti-RAB3B antibody [EPR12987] (ab177949) at 1/1000 dilution

    Lane 1 : 293T lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Fetal brain lysate
    Lane 4 : SW480 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Anti-RAB3B antibody [EPR12987] (ab177949)
    Anti-RAB3B antibody [EPR12987] (ab177949)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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