Mouse Prolactin/PRL ELISA Kit (ab214572)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 139 pg/ml
- Range: 0.562 ng/ml - 36 ng/ml
- Sample type: Cell culture extracts, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Prolactin/PRL ELISA Kit
See all Prolactin/PRL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 6% Inter-assay Sample n Mean SD CV% Overall 3 9% -
Sample type
Serum, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
139 pg/ml -
Range
0.562 ng/ml - 36 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 75 69% - 80% Tissue Extracts 102 98% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Prolactin/PRL ELISA Kit (ab214572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin/PRL protein in cell culture extracts, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Prolactin/PRL with 139 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Mouse Prolactin Capture Antibody 1 x 600µl 10X Mouse Prolactin Detector Antibody 1 x 600µl Mouse Prolactin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. -
Cellular localization
Secreted -
Alternative names
- Decidual prolactin
- GHA1
- Growth hormone A1
see all -
Database links
- Entrez Gene: 19109 Mouse
- SwissProt: P06879 Mouse
- Unigene: 1270 Mouse
Images
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Other - Mouse Prolactin/PRL ELISA Kit (ab214572)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Prolactin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike Prolactin in normal mouse serum samples.The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows: normal mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 20 ng/mL in mouse pituitary cell extract.
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Comparison of native Prolactin concentrations in pregnant and normal mouse serum.The concentrations of Prolactin were measured in three 2-fold serial dilutions starting at 25% serum samples in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Prolactin per mL of neat serum (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 6.5 ng/mL in pregnant mouse serum and 2.6 ng/mL in normal mouse serum.
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Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (ab214572)To learn more about the advantages of recombinant antibodies see here.
