Mouse Prolactin ELISA Kit (ab100736)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 27.43 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Prolactin ELISA Kit
See all Prolactin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
27.43 pg/ml - 20000 pg/mlRecovery
81 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.85 68% - 87% Serum 82.47 73% - 94% Plasma 76.78 67% - 85% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s Prolactin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Prolactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Prolactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Prolactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse Prolactin (lyophilized) 2 vials Prolactin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Prolactin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation. -
Cellular localization
Secreted -
Alternative names
- AV290867
- Lactogenic Hormone
- Luteotropic Hormone
see all -
Database links
- Entrez Gene: 19109 Mouse
- SwissProt: P06879 Mouse
- Unigene: 1270 Mouse
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse Prolactin (lyophilized) 2 vials Prolactin Microplate (12 x 8 wells) 1 unit Recombinant Mouse Prolactin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation. -
Cellular localization
Secreted -
Alternative names
- AV290867
- Lactogenic Hormone
- Luteotropic Hormone
see all -
Database links
- Entrez Gene: 19109 Mouse
- SwissProt: P06879 Mouse
- Unigene: 1270 Mouse
Images
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Sandwich ELISA - Mouse Prolactin (ab100736)
Prolactin measured in mouse biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 10-270 fold.
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Typical standard curveRepresentative standard curve using ab100736
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Typical standard curveRepresentative standard curve using ab100736
