Anti-Histone H4 (formyl K12) antibody (ab177068)
Key features and details
- Rabbit polyclonal to Histone H4 (formyl K12)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (formyl K12) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (formyl K12) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Cow, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in HeLa and NIH3T3 whole cell lysates as well as Calf Thymus Histone (CTH) and HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction) ICC/IF – HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (formyl K12) antibody (ab177068)
ab177068 staining Histone H4 (formyl K12) in HeLa cells. The cells were fixed with 100% methanol (5min) and then permeabilised using 0.1% PBS-Triton. Cells were then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab177068 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150081) at 1/1000 dilution (shown in green). AlexaFluor®594 Mouse monoclonal [DM1A] to alpha Tubulin (ab195889) - Microtubule Marker was used at a 1/200 dilution and incubated for 1h with the cells, to label Microtubules (shown in Red). The nuclear counter stain is DAPI (blue), which was added to the secondary antibody mixture.
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Western blot - Anti-Histone H4 (formyl K12) antibody (ab177068)All lanes : Anti-Histone H4 (formyl K12) antibody (ab177068) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa Nuclear Prep (0.5% Triton X-100 insoluble fraction)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at 1/10000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177068 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Western blot - Anti-Histone H4 (formyl K12) antibody (ab177068)All lanes : Anti-Histone H4 (formyl K12) antibody (ab177068) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at 1/10000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible non-specific binding)This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab177068 overnight at 4°C. Antibody binding was detected using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 790) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
