Anti-Dopamine Receptor D1 antibody (ab20066)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Dopamine Receptor D1 antibody
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D1 -
Host species
Rabbit -
Specificity
Stains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0% -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide:
MDGTGLVVERDFS
, corresponding to amino acids 9-21 of Human Dopamine Receptor D1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dopamine Receptor D1 antibody (ab20066)All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Forebrain
Lane 3 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
