Anti-Apc1 (phospho S377) antibody (ab12206)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S377)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apc1 (phospho S377) antibody
See all Apc1 primary antibodies -
Description
Rabbit polyclonal to Apc1 (phospho S377) -
Host species
Rabbit -
Specificity
ab12206 is specific for human APC1 phosphorylated at S377. Minimal reactivity is expected with the non-phosphorylated form of the protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Apc1 (phospho S377) antibody (ab12206)All lanes : Anti-Apc1 (phospho S377) antibody (ab12206) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate from asynchoronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat-anti-Rabbit IgG [H&L] MX at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDaThe blot was probed with ab12206 overnight at 4°C
While some phosphorylated APC1 is present in untreated cells, the amount of phosphorylated protein is increased arrested in mitosis (by treatment with with nocodazole).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc1 (phospho S377) antibody (ab12206)ab12206 at 5.0 µg/ml staining pancreatic cancinoma cells at 60X. This image shows moderate positive cytoplasmic and occasional nuclear staining. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
