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Anti-Adenosine A1 Receptor antibody (ab223377)

Anti-Adenosine A1 Receptor antibody (ab223377)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Adenosine A1 Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Adenosine A1 Receptor antibody
    See all Adenosine A1 Receptor primary antibodies
  • Description

    Rabbit polyclonal to Adenosine A1 Receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Adenosine A1 Receptor (C terminal).
    Database link: P30542

  • Positive control

    • WB: HeLa and HEK-293Trap cell lysates. ICC/IF: SK-N-BE cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab223377)
    Immunocytochemistry/ Immunofluorescence - Anti-Adenosine A1 Receptor antibody (ab223377)

    4% formaldehyde-fixed SK-N-BE cells stained for Adenosine A1 Receptor (green) using ab223377 at 1/100 dilution in ICC/IF. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223377 (D) Merge.

  • Western blot - Anti-Adenosine A1 Receptor antibody (ab223377)
    Western blot - Anti-Adenosine A1 Receptor antibody (ab223377)
    All lanes : Anti-Adenosine A1 Receptor antibody (ab223377) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293Trap cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13,36.5 kDa
    Observed band size: 36.5 kDa
    why is the actual band size different from the predicted?


    Exposure time: 6 minutes


    Blocking buffer: 5% skimmed milk in TBST.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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