Anti-Phosphotyrosine antibody (ab9319)
Key features and details
- Rabbit polyclonal to Phosphotyrosine
- Suitable for: ELISA, IP, WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Phosphotyrosine antibody
See all Phosphotyrosine primary antibodies -
Description
Rabbit polyclonal to Phosphotyrosine -
Host species
Rabbit -
Specificity
Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine. -
Tested applications
Suitable for: ELISA, IP, WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Phosphotyrosine conjugated to Keyhole Limpet Haemocyanin (KLH). Iodoacetylphosphotyramine KLH conjugates
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Positive control
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.01% Sodium azide
Constituent: 0.114% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphotyrosine antibody (ab9319)All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
Lane 3 : 5 ul of crude protein from HeLa cell lysate
Lane 4 : 15 ul of crude protein from HeLa cell lysate
Lysates/proteins at 2 mg/ml per lane.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phosphotyrosine antibody (ab9319)Immunohistochemistry analysis of human breast cancer with ab9319
