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Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)

Key features and details

  • Mouse monoclonal [4B11C8] to BTN2A2/BTF2
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-BTN2A2/BTF2 antibody [4B11C8]
    See all BTN2A2/BTF2 primary antibodies

  • Description

    Mouse monoclonal [4B11C8] to BTN2A2/BTF2

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
    Database link: Q8WVV5

  • Positive control

    • WB: K562 cell lysate; Human BTN2A2/BTF2 (aa 57-237) recombinant protein; BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + Human BTN2A2/BTF2 (aa 57-237) recombinant protein

    Predicted band size: 59 kDa

  • Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    All lanes : Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 59 kDa

  • Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Western blot - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + K562 cell lysate

    Predicted band size: 59 kDa

  • Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BTN2A2/BTF2 with ab233752 at 1/200 dilution (green) compared with a negative control (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
    Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)

    HeLa (human prostate adenocarcinoma cell line) cells stained for BTN2A2/BTF2 (green) using ab233752 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

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