Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752)
Key features and details
- Mouse monoclonal [4B11C8] to BTN2A2/BTF2
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-BTN2A2/BTF2 antibody [4B11C8]
See all BTN2A2/BTF2 primary antibodies -
Description
Mouse monoclonal [4B11C8] to BTN2A2/BTF2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
Database link: Q8WVV5 -
Positive control
- WB: K562 cell lysate; Human BTN2A2/BTF2 (aa 57-237) recombinant protein; BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4B11C8 -
Isotype
IgG2a -
Research areas
Images
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Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + Human BTN2A2/BTF2 (aa 57-237) recombinant protein
Predicted band size: 59 kDa
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All lanes : Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 59 kDa
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Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + K562 cell lysate
Predicted band size: 59 kDa
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BTN2A2/BTF2 with ab233752 at 1/200 dilution (green) compared with a negative control (red).
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HeLa (human prostate adenocarcinoma cell line) cells stained for BTN2A2/BTF2 (green) using ab233752 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.