Human FX ELISA kit (total FX antigen) (ab272773)
Key features and details
- Range: 0.1 ng/ml - 50 ng/ml
- Sample type: Cit plasma, EDTA Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Human FX ELISA kit (total FX antigen)
See all Factor X kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 1 20 0.71ng/ml 0.039 5.44% Sample 2 20 2.97ng/ml 0.155 5.23% Sample 3 20 12.63ng/ml 0.095 2.45% -
Sample type
EDTA Plasma, Cit plasma -
Assay type
Quantitative -
Range
0.1 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 98 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FX ELISA kit (total FX antigen) (ab272773) is intended for the quantitative determination of total Factor X antigen in human plasma.
Human Factor X will bind to the capture antibody coated on the microtiter plate. Factor X and Xa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.
Factor X standard provided is calibrated against the WHO 4th International Standard.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human FX Primary Antibody Lyophilized Vial 1 vial Anti-Rabbit Streptavidin-HRP Secondary Reagent 1 vial FX ELISA Plate 1 unit Human FX Standard Lyophilized Vial 1 vial TMB Substrate 1 x 10ml -
Research areas
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated factor Xa heavy chain
- Coagulation factor X
- F10
see all -
Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
Images
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Example data
A typical standard curve. Example only.