Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)
![Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)](https://www.abcam.com/ps/products/233/ab233771/Images/ab233771-333743-AP4376772hucolonadeno1ugmledit.jpg "Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)")
Key features and details
- Mouse monoclonal [5H10] to beta Catenin - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-beta Catenin antibody [5H10] - BSA and Azide free
See all beta Catenin primary antibodies -
Description
Mouse monoclonal [5H10] to beta Catenin - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Chicken beta Catenin aa 750-850. (Fused to a recombinant maltose binding protein).
Database link: O42486 -
Positive control
- WB: HAP1 whole cell lysate IHC-P: FFPE Human colon adenocarcinoma tissue sections.
-
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
ab233771 is a PBS-only buffer format of ab231305.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5H10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)
IHC image of beta Catenin staining in a section of formalin-fixed paraffin-embedded normal human colon adenocarcinoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab231305, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This data was developed using the same antibody clone (ab231305) in a different buffer formulation.
-
![Western blot - Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)](https://www.abcam.com/ps/products/233/ab233771/Images/ab233771-333741-ab231305AP4376772HAP1KOonly.jpg)
Western blot - Anti-beta Catenin antibody [5H10] - BSA and Azide free (ab233771)All lanes :
Lane 1 : HAP1 whole cell lysate
Lane 2 : HAP1 CTNNB1 knockout whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 85 kDaThis data was generated using the same 5H10 clone but in a different formulation (ab231305).
ab231305 was shown to specifically react with CTNNB1 (β-catenin) in wild type HAP1 cells. No band was observed when CTNNB1 (β-catenin) knockout samples were used. Wild-type and CTNNB1 (β-catenin) knockout samples were subjected to SDS-PAGE. ab231305 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone (ab231305) in a different buffer formulation.
