Anti-beta Catenin antibody, prediluted (ab15180)
Key features and details
- Rabbit polyclonal to beta Catenin, prediluted
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Catenin antibody, prediluted
See all beta Catenin primary antibodies -
Description
Rabbit polyclonal to beta Catenin, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- Tested with breast carcinoma. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: LoVo
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Catenin antibody, prediluted (ab15180)Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate
Observed band size: 92 kDa why is the actual band size different from the predicted?
ab2365 is the undiluted version of this antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody, prediluted (ab15180)ab15180 stained LoVo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15180 Undiluted overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody, prediluted (ab15180)Breast carcinoma stained with ab15180.
