Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
![Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)](https://www.abcam.com/ps/products/251/ab251536/Images/ab251536-406420-anti-e-cadherin-antibody-epr16845-34-bsa-and-azide-free-western-blot.jpg "Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16845-34] to E Cadherin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free
See all E Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR16845-34] to E Cadherin - BSA and Azide free -
Host species
Rabbit -
Specificity
E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed.
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Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251536 is the carrier-free version of ab212059. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251536 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16845-34 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)Lane 1 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/20000 dilution
Lane 2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/100000 dilution
All lanes : Mouse E-Cadherin active protein (aa1-709)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 98 kDa
Observed band size: 120,84 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis data was developed using ab212059, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)](https://www.abcam.com/ps/products/251/ab251536/Images/ab251536-403450-anti-e-cadherin-antibody-epr16845-34-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)Lanes 1-2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/5000 dilution
Lanes 3-5 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/1000 dilution
Lane 1 : Rat spleen lysate
Lane 2 : Rat serum
Lane 3 : Mouse plasma
Lane 4 : Mouse serum
Lane 5 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 & 5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 98 kDa
Observed band size: 120,84 kDa why is the actual band size different from the predicted?This data was developed using ab212059, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1, 4, and 5: 30 seconds; Lane 2: 3 minutes; Lane 3: 15 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; 11953314).
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![Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)](https://www.abcam.com/ps/products/251/ab251536/Images/ab251536-1-anti-e-cadherin-antibody-epr16845-34-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)This data was developed using ab212059, the same antibody clone in a different buffer formulation.E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab212059 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab212059 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: Mouse serum 10 µg (Input). Lane 2: ab212059 IP in mouse serum. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212059 in mouse serum. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second. -
![Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)](https://www.abcam.com/ps/products/251/ab251536/Images/ab251536-4-benefits-of-recombinant-antibodies.png)
Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
