Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16845-34] to E Cadherin - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free
    See all E Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR16845-34] to E Cadherin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed.

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251536 is the carrier-free version of ab212059. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251536 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Lane 1 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/20000 dilution
    Lane 2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/100000 dilution

    All lanes : Mouse E-Cadherin active protein (aa1-709)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 98 kDa
    Observed band size: 120,84 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab212059, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Western blot - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Lanes 1-2 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/5000 dilution
    Lanes 3-5 : Anti-E Cadherin antibody [EPR16845-34] (ab212059) at 1/1000 dilution

    Lane 1 : Rat spleen lysate
    Lane 2 : Rat serum
    Lane 3 : Mouse plasma
    Lane 4 : Mouse serum
    Lane 5 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 & 5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 2-4 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 98 kDa
    Observed band size: 120,84 kDa why is the actual band size different from the predicted?



    This data was developed using ab212059, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1, 4, and 5: 30 seconds; Lane 2: 3 minutes; Lane 3: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; 11953314).

  • Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    This data was developed using ab212059, the same antibody clone in a different buffer formulation.E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab212059 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab212059 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution Lane 1: Mouse serum 10 µg (Input). Lane 2: ab212059 IP in mouse serum. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212059 in mouse serum. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)
    Anti-E Cadherin antibody [EPR16845-34] - BSA and Azide free (ab251536)

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