Anti-beta Catenin antibody (ab2365)
Key features and details
- Rabbit polyclonal to beta Catenin
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta Catenin antibody
See all beta Catenin primary antibodies -
Description
Rabbit polyclonal to beta Catenin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
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Immunogen
Synthetic peptide within Human beta Catenin aa 750 to the C-terminus. The exact sequence is proprietary.
Database link: P35222 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Catenin antibody (ab2365)Anti-beta Catenin antibody (ab2365) at 1/25 dilution + A431 cell lysate
Predicted band size: 85 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab2365)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for beta Catenin using ab2365 at 1/250 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-beta Catenin antibody (ab2365)Beta Catenin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to beta Catenin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2365.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 85kDa; beta Catenin.
