Human ICAM3 ELISA Kit (CD50) (ab275097)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.036 ng/ml
- Range: 0.28 ng/ml - 18 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICAM3 ELISA Kit (CD50)
See all ICAM3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4% Inter-assay Sample n Mean SD CV% Serum 4 4.2% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.036 ng/ml -
Range
0.28 ng/ml - 18 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 101% - 109% Cell culture media 90 81% - 97% Hep Plasma 107 101% - 110% EDTA Plasma 102 93% - 108% Cit plasma 99 91% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ICAM3 ELISA kit (ab275097) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ICAM3 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ICAM3 with 0.036 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Intracellular adhesion molecule 3 (ICAM-3), also known as CD50, is a protein encoded by the gene ICAM3. ICAM3 belongs to the ICAM family of proteins, where all ICAM proteins are type I transmembrane glycoproteins, contain several immunoglobulin-like c2 domains, and bind to leukocyte adhesion protein (LFA-1). In addition, ICAM-3 is a ligand for integrin alpha-D/beta-2, where it contributes to apoptotic neutrophil phagocytosis by macrophages.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ICAM3 (CD50) Capture Antibody 1 x 600µl 10X Human ICAM3 (CD50) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human ICAM3 (CD50) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsUpon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD50
- CDW 50
- CDw50
see all -
Database links
- Entrez Gene: 3385 Human
- Omim: 146631 Human
- SwissProt: P32942 Human
- Unigene: 654563 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ICAM3 standard curve in Sample Diluent NS.The ICAM3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ICAM3 in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin).The concentrations of ICAM3 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 124.4 ng/mL in serum, 87.3 ng/mL in plasma (EDTA), 96.7 ng/mL in and plasma (citrate), 105 ng/mL in plasma (heparin).
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Serum from eight individual healthy ICAM3 female donors was measured in duplicate. -
Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (ab275097)To learn more about the advantages of recombinant antibodies see here.
