Recombinant human MEK5 protein (ab204150)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human MEK5 protein -
Biological activity
The specific activity is 85 nmol/min/mg.
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Purity
> 85 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQ VLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIE PLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSA ELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLD ITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLD VYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLC DFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA LGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCM RKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP -
Predicted molecular weight
77 kDa including tags -
Amino acids
1 to 448 -
Tags
GST tag N-Terminus -
Additional sequence information
BC008838
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.002% PMSF, 0.87% Sodium chloride, 0.004% DTT, 0.307% Glutathione, 0.003% EDTAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human MEK5 protein (ab204150)The specific activity of MEK5 (ab204150) was determined to be 40.4 nmol/min/mg as per activity assay protocol and was equivalent to 72 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human MEK5 protein (ab204150)SDS PAGE analysis of ab204150 -
SDS-PAGE - Recombinant human MEK5 protein (ab204150)SDS PAGE analysis of ab204150 -
Functional Studies - Recombinant human MEK5 protein (ab204150)Sample Kinase Activity Plot. ab204150 specific activity was determined to be 85 nmol/min/mg.
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SDS-PAGE - Recombinant human MEK5 protein (ab204150)SDS-PAGE analysis ofab204150. MW 77 KDa.