Human AMH ELISA Kit (ab267629)
Key features and details
- Sensitivity: 0.18 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human AMH ELISA Kit
See all AMH kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.18 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.9 71% - 144% Plasma 84 72% - 106% Cell culture media 121 97% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human AMH ELISA Kit is designed for the quantitative determination of AMH in cell culture supernatants, plasma and serum samples.
Δ Note: AMH concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for human AMH coated on a 96-well plate. Standards and samples are pipetted into the wells and AMH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AMH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AMH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human AMH coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human AMH Detection Antibody 2 vials Human AMH Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
-
Relevance
Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth. -
Cellular localization
Secreted -
Alternative names
- Anti muellerian hormone
- MIF
- MIS
see all -
Database links
- Entrez Gene: 268 Human
- Omim: 600957 Human
- SwissProt: P03971 Human
- Unigene: 112432 Human
Images
-
Standard curve
Human AMH ELISA kit (ab267629) Standard curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
