Anti-P cadherin antibody [6A9] (ab19350)
![Anti-P cadherin antibody [6A9] (ab19350)](https://www.abcam.com/ps/products/19/ab19350/Images/ab19350-339377-ICCAP439715901.jpg "Anti-P cadherin antibody [6A9] (ab19350)")
Key features and details
- Mouse monoclonal [6A9] to P cadherin
- Suitable for: ICC/IF, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-P cadherin antibody [6A9]
See all P cadherin primary antibodies -
Description
Mouse monoclonal [6A9] to P cadherin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to P cadherin. Proteins removed from A431 cell membranes by trypsinization.
-
Positive control
- ICC: A431 cells. ICC/IF: A431 cells.
-
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6A9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)
ab19350 staining P-Cadherin in A431 cells (top panel) and MCF7 (negative cell line - bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
![Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)](https://www.abcam.com/ps/products/19/ab19350/Images/ab19350-319891-ICCAP439715901.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-P cadherin antibody [6A9] (ab19350)ab19350 staining P-Cadherin in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19350 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
