Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043)
![Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043)](https://www.abcam.com/ps/products/276/ab276043/Images/ab276043-1-AntiCarbonic-anhydrase-2-CA2-antibody-001-wb-293T-cells.jpg "Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [001] to Carbonic anhydrase 2/CA2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free
See all Carbonic anhydrase 2/CA2 primary antibodies -
Description
Rabbit monoclonal [001] to Carbonic anhydrase 2/CA2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human Carbonic anhydrase 2/CA2 aa 1-260 (C terminal). NP_000058.1
Database link: P00918 -
Positive control
- WB: HEK-293T whole cell lysate. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
001 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [001] (ab276043)Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043) at 1/10000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 29 kDaDeveloped using the Odyssey technique.
Performed under reducing conditions. -
![Immunoprecipitation - Anti-Carbonic anhydrase 2/CA2 antibody [001] (ab276043)](https://www.abcam.com/ps/products/276/ab276043/Images/ab276043-2-AntiCarbonic-anhydrase-2-CA2-antibody-001-IP-293T-cells.jpg)
Immunoprecipitation - Anti-Carbonic anhydrase 2/CA2 antibody [001] (ab276043)Carbonic anhydrase 2/CA2 was immunoprecipitated from 0.5 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole Cell Lysate using 2 µL ab276043 and 15 µl of 50 % Protein G agarose.
Primary antibody ab276043 was used at 1/2000 dilution for WB.
Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution
Performed under reducing conditions. -
![Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043)](https://www.abcam.com/ps/products/276/ab276043/Images/ab276043-3-benefits-of-recombinant-antibodies.png)
Anti-Carbonic anhydrase 2/CA2 antibody [001] - BSA and Azide free (ab276043)