Anti-MEK3 antibody (ab154913)
Key features and details
- Rabbit polyclonal to MEK3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEK3 antibody
See all MEK3 primary antibodies -
Description
Rabbit polyclonal to MEK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to a region within amino acids 1-347 of Human MEK3 (Uniprot ID: P46734).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Raji and mouse liver whole cell lysates; HeLa cells; SW480 xenograft tissue.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-MEK3 antibody (ab154913) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE -
Anti-MEK3 antibody (ab154913) at 1/500 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labeling MEK3 with ab154913 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells labeling MEK3 with ab154913 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).