Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP555Y] to MEK3
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-MEK3 antibody [EP555Y]
See all MEK3 primary antibodies
Description

Rabbit monoclonal [EP555Y] to MEK3
Host species

Rabbit
Specificity

This antibody is specific for MEK3.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MEK3 aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- Jurkat, Hela, MCF-7 and K562 cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP555Y
Isotype

IgG
Research areas

Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK3knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab40836 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab40836 was shown to recognize MEK3 when MEK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK3 knockout samples were subjected to SDS-PAGE. ab40836 and ab8245 (loading control to GAPDH) were both diluted to 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MEK3 antibody [EP555Y] (ab40836)

All lanes : Anti-MEK3 antibody [EP555Y] (ab40836) at 1/20000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 39 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Anti-MEK3 antibody [EP555Y] (ab40836)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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