Anti-MEK3 (phospho S189) antibody (ab131283)
Key features and details
- Rabbit polyclonal to MEK3 (phospho S189)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK3 (phospho S189) antibody
See all MEK3 primary antibodies -
Description
Rabbit polyclonal to MEK3 (phospho S189) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human MEK3 (phospho S189) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_002747.2 -
Positive control
- WB: HeLa cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131283 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEK3 (phospho S189) antibody (ab131283)All lanes : Anti-MEK3 (phospho S189) antibody (ab131283) at 1/500 dilution
Lane 1 : HeLa cell extract untreated
Lane 2 : HeLa cell extract treated with PMA
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 (phospho S189) antibody (ab131283)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling MEK3 (phospho S189) with ab131283 at 1/50 dilution. Right panel was preincubated with blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-MEK3 (phospho S189) antibody (ab131283)Immunofluorescence analysis of methanol-fixed HeLa cells labelling MEK3 (phospho S189) with ab131283 at 1/100 dilution.
