Anti-MEK3 (phospho T222) antibody (ab79586)
Key features and details
- Rabbit polyclonal to MEK3 (phospho T222)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MEK3 (phospho T222) antibody
See all MEK3 primary antibodies -
Description
Rabbit polyclonal to MEK3 (phospho T222) -
Host species
Rabbit -
Specificity
ab79586 detects endogenous levels of MEK3 only when phosphorylated at threonine 222. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of threonine 222 (A-K-TP-M-D)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79586 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MEK3 (phospho T222) antibody (ab79586)All lanes : Anti-MEK3 (phospho T222) antibody (ab79586) at 1/500 dilution
Lane 1 : Jurkat cell extracts treated
with serum (20 %, 15 minutes)
Lane 2 : Jurkat cell extracts treated
with serum (20 %, 15 minutes) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK3 (phospho T222) antibody (ab79586)ab79586, at a 1/50 dilution, staining MEK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
