C2C12 nuclear extract lysate (ab14640)
Overview
-
Product name
C2C12 nuclear extract lysate
See all C2C12 lysates -
General notes
Cell line: C2C12 (Mouse myoblast).
We recommend aliquoting the extracts into single use fractions and then storing.
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.0087% PMSF, 0.0095% Magnesium chloride, 0.0077% DTT, 0.476% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
-
Background
C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
