Anti-CAPS1 antibody (ab32011)
Key features and details
- Rabbit polyclonal to CAPS1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CAPS1 antibody
See all CAPS1 primary antibodies -
Description
Rabbit polyclonal to CAPS1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CAPS1.
Read Abcam's proprietary immunogen policy (Peptide available as ab32785.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Target
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Relevance
CAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 8618 Human
- Entrez Gene: 27062 Mouse
- Entrez Gene: 26989 Rat
- Omim: 604667 Human
- SwissProt: Q9ULU8 Human
- SwissProt: Q80TJ1 Mouse
- SwissProt: Q62717 Rat
- Unigene: 260881 Mouse
see all -
Alternative names
- CADPS antibody
- CADPS1 antibody
- Calcium dependent secretion activator 1 antibody
see all
Images
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Western blot - Anti-CAPS1 antibody (ab32011)All lanes : Anti-CAPS1 antibody (ab32011) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lane 5 : Brain (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Lane 3 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 146 kDaab32011 is detecting a doublet at the expected molecular weight and both of these bands are blocked by the immunizing peptide. We are unsure what this doublet represents and would be interested in any feedback.
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Immunocytochemistry/ Immunofluorescence - Anti-CAPS1 antibody (ab32011)ICC/IF image of ab32011 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32011, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (1)
ab32011 has been referenced in 1 publication.
- Wu B et al. Exosomes isolated from CAPS1-overexpressing colorectal cancer cells promote cell migration. Oncol Rep 42:2528-2536 (2019). PubMed: 31638236
Images
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Western blot - Anti-CAPS1 antibody (ab32011)All lanes : Anti-CAPS1 antibody (ab32011) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lane 5 : Brain (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lane 6 : Heart (Mouse) Tissue Lysate with CAPS1 peptide (ab32785) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Lane 3 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 146 kDaab32011 is detecting a doublet at the expected molecular weight and both of these bands are blocked by the immunizing peptide. We are unsure what this doublet represents and would be interested in any feedback.
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Immunocytochemistry/ Immunofluorescence - Anti-CAPS1 antibody (ab32011)
ICC/IF image of ab32011 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32011, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
