Anti-beta Catenin (phospho Y142) antibody (ab27798)
Key features and details
- Rabbit polyclonal to beta Catenin (phospho Y142)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-beta Catenin (phospho Y142) antibody
See all beta Catenin primary antibodies -
Description
Rabbit polyclonal to beta Catenin (phospho Y142) -
Host species
Rabbit -
Specificity
The antibody detects beta Catenin on SDS PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate treated cell lines, such as A431 and human endothelial cells. Immunogen sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma Catenin. These human sequences are highly conserved in rat and mouse beta Catenins. Batches could vary in the level phospho Y133 of gamma catenin recognition. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Recombinant fragment -
Immunogen
Phospho beta Catenin (Tyr 142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta Catenin.
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General notes
Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)Lanes 1-2 : anti beta catenin
Lanes 3-4 : Anti-beta Catenin (phospho Y142) antibody (ab27798) at 1/500 dilution
Lanes 1 & 3 : Hct116 src transformed cells serum starved overnight.
Lanes 2 & 4 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.
Lysates/proteins at 20 µg per lane.
Predicted band size: 86 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature. -
Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)ICC/IF labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin (left panel) and ab27798 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798) Image courtesy of an Abreview submitted by Judit Herreros.ab27798 staining beta Catenin (phospho Y142) in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were pre-treated with pervanadate for 15 minutes before being fixed in paraformaldehyde and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 8 hours at 4°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) ab150076) used at a 1/500 dilution. Hoestch was used to stain the cell nuclei (blue).
