Anti-Desmocollin 1 antibody [EPR8220] (ab150382)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8220] to Desmocollin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Desmocollin 1 antibody [EPR8220]
See all Desmocollin 1 primary antibodies -
Description
Rabbit monoclonal [EPR8220] to Desmocollin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide within Human Desmocollin 1 aa 850 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q08554 -
Positive control
- WB: A375 and A431 whole cell lysates. Human skin tissue lysate. Mouse brain, skin, and kidney tissue lysates. Rat brain and kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8220 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Desmocollin 1 antibody [EPR8220] (ab150382) at 1/10000 dilution (purified)
Lane 1 : A375 (Human malignant melanoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : Human skin lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat brain lysate
Lane 8 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDaWe are unsure about the extra bands. They might be the deglycosylated forms as described in PMID: 25918164.
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