Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CTNNA3 antibody [EPR18307] (ab184916)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18307] to CTNNA3
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Dog, Human

Product name

Anti-CTNNA3 antibody [EPR18307]
See all CTNNA3 primary antibodies
Description

Rabbit monoclonal [EPR18307] to CTNNA3
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Dog Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293, C2C12, Rat1, C6, PC-12, NIH/3T3 and MDCK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain and heart lysates. IHC/P: Human cardiac muscle and colon cancer tissues; Mouse and rat cardiac muscle tissues. IP: HEK293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18307
Isotype

IgG
Research areas

Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 3 : Rat1 (Rat fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and membrane staining on tumor cells of Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Mouse cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining of normal Human cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

All lanes : Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/5000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CTNNA3 with ab184916 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Intercalated disks staining on Rat cardiac muscle is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CTNNA3 antibody [EPR18307] (ab184916)

Anti-CTNNA3 antibody [EPR18307] (ab184916) at 1/50000 dilution + MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 100 kDa
Observed band size: 100 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-CTNNA3 antibody [EPR18307] (ab184916)

CTNNA3 was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab184916 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab184916 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate, 10 µg (Input).
Lane 2: ab184916 IP in HEK-293 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184916 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
Anti-CTNNA3 antibody [EPR18307] (ab184916)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTNNA3 antibody [EPR18307] (ab184916)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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