Anti-DOK1 (phospho Y362) antibody (ab75742)
Key features and details
- Rabbit polyclonal to DOK1 (phospho Y362)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK1 (phospho Y362) antibody
See all DOK1 primary antibodies -
Description
Rabbit polyclonal to DOK1 (phospho Y362) -
Host species
Rabbit -
Specificity
ab75742 endogenous levels of DOK1 only when phosphorylated at tyrosine 362. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A Synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 362 (P-I-YP-D-E).
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Positive control
- Human breast carcinoma tissue and extracts from Jurkat cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab75742 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK1 (phospho Y362) antibody (ab75742)ab75742, at a 1/50 dilution, staining DOK1 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.
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Western blot - Anti-DOK1 (phospho Y362) antibody (ab75742)All lanes : Anti-DOK1 (phospho Y362) antibody (ab75742) at 1/500 dilution
Lane 1 : Jurkat cell extracts with immunising peptide
Lane 2 : Jurkat cell extracts
Predicted band size: 52 kDa
Observed band size: 52 kDa
