Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD31 antibody [RM0032-1D12] (ab56299)

Key features and details

  • Rat monoclonal [RM0032-1D12] to CD31
  • Suitable for: IHC-P
  • Reacts with: Mouse
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CD31 antibody [RM0032-1D12]
    See all CD31 primary antibodies

  • Description

    Rat monoclonal [RM0032-1D12] to CD31

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to CD31. Mouse endothelial membrane protein

  • Positive control

    • IHC-P: Mouse kidney, lung, heart and liver tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299) Yoshida et al PLoS One. 2016 Aug 2;11(8):e0160330. doi: 10.1371/journal.pone.0160330. eCollection 2016. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Tumor vascularization and VEGF in ascites.

    (A) ID8, ID8-c-MYC, or ID8-KRAS cells (2 × 106) were intra-peritoneal injected into C57/BL6 mice. Mice were sacrificed when the body weight reached 23 g. Disseminations were obtained from mice when sacrificed. Tumor vascularization of each dissemination was assessed using CD31 immunohistochemistry (ab56299).

    Scale bars represent 200 μm and 50 μm at low and high magnification, respectively.

    Paraffin sections (4-μm-thick) of the biggest tumor sections from ID8, ID8-c-MYC, and ID8-KRAS mice were dewaxed in xylene and rehydrated through graded ethanol to water. Antigens were retrieved by boiling in 10 mM citrate buffer (pH 6.0) for 30 min. The cooled sections were incubated in a peroxidase blocking solution for 10 min to quench endogenous peroxidase. Sections were incubated in a protein blocking solution at room temperature for 10 min to block non-specific binding. Sections were then stained for Ki67 using rabbit monoclonal antibody against mouse Ki67, CD31 using a rat monoclonal antibody against mouse CD31 (ab56299, Abcam 1:100 dilution).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299) Vasilopoulou et al PLoS One. 2013 Jun 12;8(6):e65402. doi: 10.1371/journal.pone.0065402. Print 2013. Fig 8. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Proliferation and cell number in male wild-type (+/y) and male Mct8 knockout (−/y) placentae.

    (Panel C): Representative picture demonstrating CD31 immunoreactivity (marker of endothelial cells represented by brown staining) in the mouse placenta at E18.5. The nuclei were counterstained using hematoxylin. Endothelial cells are indicated by arrows and trophoblast cells by arrowheads.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)

    ab56299 at 1/400 staining CD31 in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)

    ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse liver tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)

    ab56299 at 1/400 staining CD31 in LPS treated mouse lung tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM0032-1D12] (ab56299)

    ab56299 at 1/400 dilution, staining CD31 in LPS treated mouse heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

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