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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-Syk antibody (ab245328)

Anti-Syk antibody (ab245328)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Syk
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Syk antibody
    See all Syk primary antibodies
  • Description

    Rabbit polyclonal to Syk
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide within Human Syk aa 350-400. The exact sequence is proprietary. NP_003168.2
    Database link: P43405

  • Positive control

    • WB: Ramos whole cell lysate. IP: Ramos whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245328 was affinity purified using an epitope specific to Syk immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • Mast Cells

Images

  • Western blot - Anti-Syk antibody (ab245328)
    Western blot - Anti-Syk antibody (ab245328)
    All lanes : Anti-Syk antibody (ab245328) at 0.1 µg/ml

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
    Lane 2 : Ramos whole cell lysate at 15 µg
    Lane 3 : Ramos whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • Immunoprecipitation - Anti-Syk antibody (ab245328)
    Immunoprecipitation - Anti-Syk antibody (ab245328)

    Syk was immunoprecipitated from Ramos (human Burkitt's lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245328 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab245328 at 1 µg/ml.

    Lane 1: ab245328 (Lot 1) IP in Ramos whole cell lysate.

    Lane 2: ab245328 (Lot 2) IP in Ramos whole cell lysate.

    Lane 3: Control IgG IP in Ramos whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepated in NETN buffer.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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