Recombinant human VEGF Receptor 1 protein (ab54346)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human VEGF Receptor 1 protein
See all VEGF Receptor 1 proteins and peptides -
Biological activity
The activity of sVEGFR-1 was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.
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Purity
> 90 % SDS-PAGE.
Purified protein was visualised by Silver stain. -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SGSKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERL SITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAI YIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPL DTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHR QTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNK RASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNT SVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGL PATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIV NVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHN HSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVV ADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVNLEKMPTEGEDLK LSCTVNKFLYRDVTWILLRTVNNRTMHYSISKQKMAITKEHSITLNLTIM NVSLQDSGTYACRARNVYTGEEILQKKEITIRGEHCNKKAVFSRISKFKS TRNDCTTQSNVKH -
Amino acids
25 to 688 -
Additional sequence information
VEGF Receptor 1 isoform 2 without the signal peptide
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Preparation and Storage
Images
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Functional Studies - Recombinant human VEGF Receptor 1 protein (ab54346)
FLT-1 Sandwich-ELISA using recombinant human soluble FLT-1 as standard. Mouse anti-human FLT-1 was used as capture antibody, Biotinylated rabbit anti-human FLT-1 was used for detection.
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SDS-PAGE - Recombinant human VEGF Receptor 1 protein (ab54346)SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie stain. -
Functional Studies - Recombinant human VEGF Receptor 1 protein (ab54346)Inhibition of the VEGF165-induced proliferation in HUVECs by soluble VEGFR-1/Flt-1. VEGF165 (10ng/ml) was preincubated with increasing amounts of sVEGFR-1/Flt-1 for 1h and then added to the cells.
