Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22639-68] to Asialoglycoprotein Receptor 1/HL-1
Suitable for: WB, ICC/IF, IHC-P, IP
Reacts with: Human

Product name

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68]
See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies
Description

Rabbit monoclonal [EPR22639-68] to Asialoglycoprotein Receptor 1/HL-1
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2 and Huh7 cell lysates whole cell lysate. Human liver tissue lysate. IP: HepG2 whole cell lysates IHC-P Human liver and liver cancer tissue ICC/IF: HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22639-68
Isotype

IgG
Research areas

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261) at 1/1000 dilution

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 33 kDa
Observed band size: 40-50 kDa
why is the actual band size different from the predicted?

The diffuse bands between 40-50 kDa are likely due to glycosylation.

The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079, 22898823).

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane 1: 5.5 seconds; Lane 2: 15 seconds.
Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261) at 1/1000 dilution + Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

The diffuse bands between 40-50 kDa are likely due to glycosylation.

The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079).

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver cancer (PMID:23979840,26422581,27241665).No staining in human colon (PMID:23979840). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Negative control: No staining in human colon (PMID:23979840).

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Immunofluorescent analysis of 100% Methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Asialoglycoprotein Receptor 1/HL-1 with at 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Methanol fixation is preferred.

ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg human liver lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 at 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human liver lysate 10μg.

Lane 2: ab254261 IP in human liver lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in human liver lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second
Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2: ab254261 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254261 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

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