Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)
Key features and details
- Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody
See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies -
Description
Rabbit polyclonal to Asialoglycoprotein Receptor 1/HL-1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab98248) -
Positive control
- This antibody gave a positive signal in HepG2 whole cell lysate and FFPE human liver tissue sections.
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General notes
Previously labelled as Asialoglycoprotein Receptor 1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab88042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 33 kDa). Target
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Function
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. -
Tissue specificity
Expressed exclusively in hepatic parenchymal cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on a cytoplasmic Ser residue. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 432 Human
- Entrez Gene: 100172364 Orangutan
- Omim: 108360 Human
- SwissProt: P07306 Human
- SwissProt: Q5RBQ8 Orangutan
- Unigene: 12056 Human
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Alternative names
- ASGP-R 1 antibody
- ASGPR 1 antibody
- ASGPR antibody
see all
Images
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml
Lane 1 : Human Liver Tissue Lysate
Lane 2 : HepG2 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 44,48 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk/TBST before being incubated with ab88042 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThe predicted molecular weight of Human Asialoglycoprotein Receptor 1 / HL-1 is 33 kDa (SwissProt), however we expect to observe a banding pattern at 46 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)IHC image of ab88042 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88042, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (5)
ab88042 has been referenced in 5 publications.
- Mancio-Silva L et al. Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers. Cell Metab 29:727-735.e3 (2019). PubMed: 30840913
- Workinger JL et al. Systemically Administered Plant Recombinant Holo-Intrinsic Factor Targets the Liver and is not Affected by Endogenous B12 levels. Sci Rep 9:12269 (2019). PubMed: 31439908
- Casey CA et al. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules 8:N/A (2018). PubMed: 30453527
- Zhang D et al. Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with (99m)Tc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice. Sci Rep 6:25377 (2016). PubMed: 27150943
- Casey CA et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Alcohol Clin Exp Res 40:2573-2590 (2016). PubMed: 27748959
Images
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml
Lane 1 : Human Liver Tissue Lysate
Lane 2 : HepG2 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 44,48 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Milk/TBST before being incubated with ab88042 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThe predicted molecular weight of Human Asialoglycoprotein Receptor 1 / HL-1 is 33 kDa (SwissProt), however we expect to observe a banding pattern at 46 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab88042)IHC image of ab88042 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88042, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
