Pig Fibrinogen ELISA Kit (ab205090)
Key features and details
- Sensitivity: 1.713 ng/ml
- Range: 12.5 ng/ml - 400 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Overview
-
Product name
Pig Fibrinogen ELISA Kit
See all Fibrinogen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.713 ng/ml -
Range
12.5 ng/ml - 400 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 12.5ng/ml - 400ng/ml -
Assay time
1h 10m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Pig -
Product overview
Fibrinogen in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibrinogen protein in pig biological samples.
-
Notes
In this assay the Fibrinogen present in samples reacts with the anti-Fibrinogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP Conjugated Enzyme Antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentration 1 x 50ml Anti-Pig Fibrinogen ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Pig Fibrinogen Calibrator (Lyophilized) 1 vial Stop Solution 1 x 12ml -
Research areas
-
Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- FGA
- FGB
- FGG
see all -
Database links
- Entrez Gene: 100514354 Pig
- Entrez Gene: 403164 Pig
- SwissProt: P14477 Pig
- SwissProt: P14460 Pig