Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22254-115] to Plasminogen
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Plasminogen antibody [EPR22254-115]
    See all Plasminogen primary antibodies

  • Description

    Rabbit monoclonal [EPR22254-115] to Plasminogen

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse plasma and serum; Mouse liver lysate. IHC-P: Mouse liver and kidney tissues; Human liver tissue. IP: Mouse liver lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Plasminogen with ab242329 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver (PMID: 11916082) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Immunoprecipitation - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

    Plasminogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab242329 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242329 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab242329 IP in mouse liver lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242329 in mouse liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Western blot - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Western blot - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    All lanes : Anti-Plasminogen antibody [EPR22254-115] (ab242329) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human serum
    Lane 3 : Mouse plasma
    Lane 4 : Mouse serum
    Lane 5 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
    Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 91 kDa



    Blocking/Dilutiom buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1, 2, 3 and 4: 1 second; Lanes 5: 50 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Plasminogen with ab242329 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human liver (PMID: 11916082) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Plasminogen with ab242329 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on plasma in mouse kidney (PMID: 11916082) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Plasminogen antibody [EPR22254-115] (ab242329)
    Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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