Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22254-115] to Plasminogen
Suitable for: IHC-P, IP, WB
Reacts with: Mouse, Human

Product name

Anti-Plasminogen antibody [EPR22254-115]
See all Plasminogen primary antibodies
Description

Rabbit monoclonal [EPR22254-115] to Plasminogen
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human and mouse plasma and serum; Mouse liver lysate. IHC-P: Mouse liver and kidney tissues; Human liver tissue. IP: Mouse liver lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22254-115
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Plasminogen with ab242329 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver (PMID: 11916082) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Plasminogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab242329 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242329 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Mouse brain lysate 10 µg (Input).

Lane 2: ab242329 IP in mouse liver lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242329 in mouse liver lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Western blot - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

All lanes : Anti-Plasminogen antibody [EPR22254-115] (ab242329) at 1/1000 dilution

Lane 1 : Human plasma
Lane 2 : Human serum
Lane 3 : Mouse plasma
Lane 4 : Mouse serum
Lane 5 : Mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 91 kDa

Blocking/Dilutiom buffer: 5% NFDM/TBST.

Exposure times: Lanes 1, 2, 3 and 4: 1 second; Lanes 5: 50 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Plasminogen with ab242329 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human liver (PMID: 11916082) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Plasminogen with ab242329 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on plasma in mouse kidney (PMID: 11916082) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Plasminogen antibody [EPR22254-115] (ab242329)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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