Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Plasminogen antibody [EPR22406-159] (ab245247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22406-159] to Plasminogen
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Plasminogen antibody [EPR22406-159]
    See all Plasminogen primary antibodies

  • Description

    Rabbit monoclonal [EPR22406-159] to Plasminogen

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse serum and plasma; Mouse liver lysate. IP: Mouse liver lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
    Western blot - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
    All lanes : Anti-Plasminogen antibody [EPR22406-159] (ab245247) at 1/1000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse plasma
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 91 kDa
    Observed band size: 97 kDa
    why is the actual band size different from the predicted?



    Exposure time : Lane 1:15 seconds; Lane 2:5 seconds; Lane 3:3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] (ab245247)
    Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] (ab245247)

    Plasminogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab245247 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245247 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse liver lysate 10 µg (Input). 

    Lane 2: ab245247 IP in mouse liver lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245247 in mouse liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Plasminogen antibody [EPR22406-159] (ab245247)
    Anti-Plasminogen antibody [EPR22406-159] (ab245247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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