Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22199-203] to Angiotensinogen - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free
    See all Angiotensinogen primary antibodies

  • Description

    Rabbit monoclonal [EPR22199-203] to Angiotensinogen - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse kidney tissue.

  • General notes

    Ab238173 is the carrier-free version of ab229005. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238173 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the Leydig cells of mouse testis (PMID: 20361351) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229005).

  • Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)
    Immunoprecipitation - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)

    Angiotensinogen was immunoprecipitated from 0.35 mg mouse liver tissue lysates with ab229005 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229005 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: Mouse liver lysate (10 μg) (Input).
    Lane 2: ab29005 IP in mouse liver lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229005 in mouse liver lysate (-).

    Blocking/D ilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229005).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for Angiotensinogen with ab229005 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in the proximal tubules of mouse kidney (PMID: 7648615) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab229005).

  • Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)
    Anti-Angiotensinogen antibody [EPR22199-203] - BSA and Azide free (ab238173)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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