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Rat Tissue Plasminogen Activator ELISA Kit (TPA) (ab198510)

Key features and details

  • Sensitivity: 0.043 ng/ml
  • Range: 0.05 ng/ml - 50 ng/ml
  • Sample type: Other biological fluids, Plasma
  • Detection method: Colorimetric
  • Assay type: Quantitative
  • Reacts with: Rat

Overview

  • Product name

    Rat Tissue Plasminogen Activator ELISA Kit (TPA)
    See all PAI1 + tPA kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Known Conc 20 0.8ng/ml 0.034 4.28%
    Known Conc 20 4.25ng/ml 0.225 5.29%
    Known Conc 20 13.2ng/ml 0.553 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Known Conc 10 1.37ng/ml 0.06 4.71%
    Known Conc 10 4.8ng/ml 0.34 7.13%
    Known Conc 10 13.3ng/ml 0.75 5.65%

  • Sample type

    Plasma, Other biological fluids

  • Assay type

    Quantitative

  • Sensitivity

    0.043 ng/ml

  • Range

    0.05 ng/ml - 50 ng/ml

  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Spike 99 93% - 107%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Rat
    Does not react with: Sheep, Rabbit, Horse, Dog, Human

  • Product overview

    Abcam’s Tissue Plasminogen Activator (TPA) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active tissue Plasminogen Activator (TPA) in rat plasma and other biological fluids.

     

    Functionally active rat TPA will form a covalent complex with the biotinylated Human PAI-1, which is bound to the avidin on the plate. Only free active TPA will react with the PAI-1 bound to the plate. After appropriate washing steps, polyclonal anti-TPA primary antibody binds to the captured TPA. Excess antibody is washed away and bound primary antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB substrate is used for color development at 450nm. The amount of color development is directly proportional to the concentration of active TPA in the sample.

  • Platform

    Microplate

Properties

Images

  • Standard Curve
    Standard Curve

    Full range standard curve using ab198510 Tissue Plasminogen Activator (TPA) Rat ELISA Kit.

  • Standard Curve
    Standard Curve

    Linear range standard curve using ab198510 Tissue Plasminogen Activator (TPA) Rat ELISA Kit.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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