Mouse uPA ELISA Kit (ab245727)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 31.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse uPA ELISA Kit
See all uPA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 2.8% Inter-assay Sample n Mean SD CV% serum 3 3.5% -
Sample type
Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
31.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 112 101% - 124% Serum 109 103% - 118% Cell culture media 95 91% - 99% Hep Plasma 110 105% - 114% EDTA Plasma 113 108% - 120% Cit plasma 106 104% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse uPA ELISA Kit (ab245727) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of uPA protein in cit plasma, edta plasma, hep plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse uPA with 31.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse uPA Capture Antibody 1 x 600µl 10X Mouse uPA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse uPA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR). -
Alternative names
- ATF
- BDPLT5
- EC 3.4.21.73
see all -
Database links
- Entrez Gene: 18792 Mouse
- SwissProt: P06869 Mouse
- Unigene: 4183 Mouse
Images
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Other - Mouse uPA ELISA Kit (ab245727)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse uPA standard curve in Sample Diluent NS.The uPA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native uPA in mouse serum, plasma samples.The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 7166 pg/mL in serum, 6222 pg/mL in plasma (citrate) and 7060 pg/mL in plasma (EDTA), and 4907 pg/mL in plasma (heparin). -
Interpolated concentrations of native uPA in mouse urine.The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 272.6 ng/mL in urine. -
Interpolated concentrations of spiked uPA in cell culture media.The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Sandwich ELISA - Mouse uPA ELISA Kit (ab245727)To learn more about the advantages of recombinant antibodies see here.
