Anti-PAI1 antibody (ab31280)
Key features and details
- Goat polyclonal to PAI1
- Suitable for: Sandwich ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAI1 antibody
See all PAI1 primary antibodies -
Description
Goat polyclonal to PAI1 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms. -
Tested applications
Suitable for: Sandwich ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee
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Immunogen
Synthetic peptide:
C-GFKIDDKGMAPALRH
, corresponding to Internal sequence amino acids 86-100 of Human PAI1 -
Positive control
- Human duodenum, liver and lung lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAI1 antibody (ab31280)Anti-PAI1 antibody (ab31280) at 0.1 µg/ml + Human Liver lysate at 35 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280) This image is courtesy of an anonymous Abreviewab31280, at 1/1000, staining plasminogen activator inhibitor 1 (PAI1) in human MDA-MB-231 cells grown in mice by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed and blocked in 10% casein for 30 minutes at 22°C and then incubated with ab31280, for 17 hours at 4°C. Horse polyclonal to goat IgG, diluted 1/200, was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody (ab31280)ab31280 (4µg/ml) staining of paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. -
Sandwich ELISA - Anti-PAI1 antibody (ab31280)ab31280 (1.5ug/ml) used as the reporter with a capture rabbit antibody (2.5ug/ml).
