Human HC-II ELISA Kit (ab277402)
Key features and details
- Sensitivity: 1.23 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human HC-II ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.23 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 118.5 89% - 149% Serum 116.4 83% - 145% Plasma 117.1 89% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human HC-II ELISA Kit (ab277402) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HC-II in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human HC-II coated on a 96-well plate. Standards and samples are pipetted into the wells and Human HC-II present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human HC-II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human HC-II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human HC-II Antibody 2 vials Anti-Human HC-II coated Microplate 1 unit Human HC-II standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. -
Tissue specificity
Expressed predominantly in liver. Also present in plasma. -
Involvement in disease
Thrombophilia due to heparin cofactor 2 deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition. -
Post-translational
modificationsPhosphorylation sites are present in the extracellular medium. - Information by UniProt
-
Alternative names
- D22S673
- HC II
- HC-II
see all -
Database links
- Entrez Gene: 3053 Human
- Omim: 142360 Human
- SwissProt: P05546 Human
- Unigene: 474270 Human
Images
-
Human HC-II ELISA kit (ab277402) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.