Recombinant human Sonic Hedgehog protein (ab123773)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Sonic Hedgehog protein
See all Sonic Hedgehog proteins and peptides -
Biological activity
Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED50, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml. -
Purity
> 95 % SDS-PAGE.
Purity is greater than 97% as determined by HPLC and SDS-PAGE. Endotoxin Level: -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
IIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG -
Predicted molecular weight
20 kDa -
Amino acids
25 to 197
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
pH: 7.50
Constituent: 0.14% Sodium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionWhen reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Upon reconstitution the material should be aliquoted and frozen at -20 °C. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
