Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)
Key features and details
- Rabbit polyclonal to Carbonic anhydrase 2/CA2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic anhydrase 2/CA2 antibody
See all Carbonic anhydrase 2/CA2 primary antibodies -
Description
Rabbit polyclonal to Carbonic anhydrase 2/CA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Carbonic anhydrase 2/CA2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab92423) -
Positive control
- WB: HAP1, HEK293T, A431 and Caco-2 cell lysates; Human bone marrow, small intestine and kidney tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseSheepRabbitCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Target
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Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
Involvement in disease
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 280740 Cow
- Entrez Gene: 760 Human
- Entrez Gene: 12349 Mouse
- Entrez Gene: 101113341 Sheep
- Omim: 611492 Human
- SwissProt: P00921 Cow
- SwissProt: P00918 Human
- SwissProt: P00920 Mouse
see all -
Alternative names
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
Images
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Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CA2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab82559 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab82559 Anti-Carbonic anhydrase 2/CA2 antibody was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab82559 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HEk293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab82559 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab82559 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. ab82559 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute
Datasheets and documents
References (1)
ab82559 has been referenced in 1 publication.
- Roossink F et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). PubMed: 22323184
Images
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Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CA2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab82559 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab82559 Anti-Carbonic anhydrase 2/CA2 antibody was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab82559 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HEk293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab82559 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab82559 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. ab82559 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Anti-Carbonic anhydrase 2/CA2 antibody (ab82559)All lanes : Anti-Carbonic anhydrase 2/CA2 antibody (ab82559) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute
