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Anti-Mouse IgG antibody [RM104] (ab190475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM104] to Mouse IgG
  • Suitable for: WB, ELISA
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Mouse IgG antibody [RM104]
    See all Mouse IgG primary antibodies

  • Description

    Rabbit monoclonal [RM104] to Mouse IgG

  • Host species

    Rabbit

  • Specificity

    This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG and rat IgG. It may cross react to goat IgG. The Fc region of this antibody has been engineered to eliminate the binding of Fc receptor.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human

  • Immunogen

    Recombinant full length protein corresponding to Mouse IgG .
    Database link: 16017

  • Positive control

    • Mouse IgG

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    RM104

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Mouse IgG antibody [RM104] (ab190475)
    Western blot - Anti-Mouse IgG antibody [RM104] (ab190475)
    All lanes : Anti-Mouse IgG antibody [RM104] (ab190475) at 0.2 µg/ml

    Lane 1 : Mouse IgG1 (non-reduced)
    Lane 2 : Mouse IgG1 (reduced)
    Lane 3 : Mouse IgG2a (non-reduced)
    Lane 4 : Mouse IgG2a (reduced)
    Lane 5 : Mouse IgG2b (non-reduced)
    Lane 6 : Mouse IgG2b (reduced)
    Lane 7 : Mouse IgG3 (non-reduced)
    Lane 8 : Mouse IgG3 (reduced)
    Lane 9 : Mouse IgM (non-reduced)
    Lane 10 : Mouse IgM (reduced)
    Lane 11 : Mouse IgA (non-reduced)
    Lane 12 : Mouse IgA (reduced)
    Lane 13 : Mouse IgE (non-reduced)
    Lane 14 : Mouse IgE (reduced)

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 38 kDa

  • ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)
    ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)

    ELISA titre of ab190475 mouse IgG. Plate was coated with 2 - 0.05 µg mouse IgG and a serial dilution of ab190475 (0.2-0.003 µg/ml) used as the primary antibody followed by alkaline phosphatase anti-rabbit IgG.

  • ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)
    ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)

    ELISA analysis of ab190475 at 10 - 200 ng/mL  using 50 ng/well of different immunoglobulins and an alkaline phosphatase conjugated anti-rabbit IgG.

  • Anti-Mouse IgG antibody [RM104] (ab190475)
    Anti-Mouse IgG antibody [RM104] (ab190475)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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