Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Mouse IgG antibody [RM104] (ab190475)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RM104] to Mouse IgG
Suitable for: WB, ELISA
Reacts with: Mouse

Product name

Anti-Mouse IgG antibody [RM104]
See all Mouse IgG primary antibodies
Description

Rabbit monoclonal [RM104] to Mouse IgG
Host species

Rabbit
Specificity

This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG and rat IgG. It may cross react to goat IgG. The Fc region of this antibody has been engineered to eliminate the binding of Fc receptor.
Tested applications

Suitable for: WB, ELISAmore details
Species reactivity

Reacts with: Mouse
Does not react with: Rat, Human
Immunogen

Recombinant full length protein corresponding to Mouse IgG .
Database link: 16017
Positive control
- Mouse IgG

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

RM104
Isotype

IgG
Research areas

Western blot - Anti-Mouse IgG antibody [RM104] (ab190475)

All lanes : Anti-Mouse IgG antibody [RM104] (ab190475) at 0.2 µg/ml

Lane 1 : Mouse IgG1 (non-reduced)
Lane 2 : Mouse IgG1 (reduced)
Lane 3 : Mouse IgG2a (non-reduced)
Lane 4 : Mouse IgG2a (reduced)
Lane 5 : Mouse IgG2b (non-reduced)
Lane 6 : Mouse IgG2b (reduced)
Lane 7 : Mouse IgG3 (non-reduced)
Lane 8 : Mouse IgG3 (reduced)
Lane 9 : Mouse IgM (non-reduced)
Lane 10 : Mouse IgM (reduced)
Lane 11 : Mouse IgA (non-reduced)
Lane 12 : Mouse IgA (reduced)
Lane 13 : Mouse IgE (non-reduced)
Lane 14 : Mouse IgE (reduced)

Lysates/proteins at 0.02 µg per lane.

Predicted band size: 38 kDa
ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)

ELISA titre of ab190475 mouse IgG. Plate was coated with 2 - 0.05 µg mouse IgG and a serial dilution of ab190475 (0.2-0.003 µg/ml) used as the primary antibody followed by alkaline phosphatase anti-rabbit IgG.
ELISA - Anti-Mouse IgG antibody [RM104] (ab190475)

ELISA analysis of ab190475 at 10 - 200 ng/mL using 50 ng/well of different immunoglobulins and an alkaline phosphatase conjugated anti-rabbit IgG.
Anti-Mouse IgG antibody [RM104] (ab190475)

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